Wednesday, March 3rd, 2021


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Investigation of the Effects of Carvacrol on Experimental Ischemia/Reperfusion Model of Rat Ovaries by Immunohistochemistry
Authors:  Nurullah Peker, M.D., Ugur Deger, M.D., and Fırat Asır, Ph.D.
  Objective: Ovarian torsion is a gynecologic problem that presents as acute lower abdominal pain. As a result, blood flow to the ovaries decreases and ischemia develops. Many medications have been used to treat ovarian torsion, including carvacrol. We conducted an experimental ischemia/reperfusion (torsion-detorsion) model using rats to observe the effects of carvacrol on ovarian torsion by immunohistochemical study.
Study Design:
Thirty-two female Wistar rats were randomly categorized into 4 groups (8 rats per group): control group, ischemia group, ischemia/reperfusion group, and ischemia/reperfusion+carvacrol–treated group. Ischemia was created by sealing the left ovaries for 3 hours, followed by 3-hour reperfusion. For the ischemia/reperfusion+carvacrol–treated group, 100 mg/kg carvacrol was administered orally in 2 mL 0.9% NaCl after the reperfusion. All animals were sacrificed, and ovarian tissues were dissected for routine paraffin wax embedding tissue protocol.
Control groups showed normal ovarian histological structures. In the ischemia group, hyperplastic granulosa cell vascular dilation, severe hemorrhage, and inflammation were observed. The ischemia/reperfusion group showed edema, inflammation, congestion, degenerated follicles, and cells with pyknotic nuclei. In the ischemia/reperfusion+carvacrol group, degenerative changes and vascular pathologies were decreased in ovarian tissues. Endothelin-1 (ET-1) was expressed in degenerated follicles and vascular endothelial cells in the ischemia and ischemia/reperfusion group. Expres-sion of ET-1 was decreased in follicular cells but negative in stromal and luteal cells in the ischemia/reperfusion+carvacrol group. ADAMTS-5 expression was positive in degenerated follicles, apoptotic cells, and inflammatory cells in the ischemia and ischemia/reperfusion groups. In the ischemia/reperfusion+carvacrol group, corona cells and a few inflammatory cells around vessels showed positive ADAMTS-5 expression.
ET-1 can induce angiogenic development with the decrease of degenerative development and inflammation in the carvacrol group. ADAMTS-5 molecule may be a marker of cellular structure and extracellular matrix development in different stages of development of ovarian cells and follicles in ischemia and oxygen deficiency caused by ischemia reperfusion.
Keywords:  ADAMTS-5; carvacrol; detorsion; endothelin-1; ischemia; ischemia-reperfusion injury; ovarian torsion; rats, Wistar; reperfusion; reperfusion injury; torsion abnormality
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