Wednesday, June 29th, 2022


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Investigation of Precore/Core Mutation of Hepatitis B Virus with Line Probe Immune Assay Method
Authors:  Erdal Ozbek, M.D., Hakan Temiz, M.D., Nida Ozcan, M.D., and Tuncer Ozekinci, M.D.
  Objective: The expression of hepatitis B e antigen (HBeAg) is reduced or totally inhibited as a result of mutations in the precore/core region of the hepatitis B virus (HBV) genome, but its clinical significance has not been yet fully understood. In this study, we aimed to investigate precore/core mutation in serum samples of patients followed with a chronic hepatitis B infection diagnosis and whose HBeAg was found to be negative on ELISA test.
Study Design:
Among the chronic hepatitis B patients followed in our hospital, serum samples of 111 patients with HBeAg negative were included in the study. In the serums of the patients whose HBV DNA were found positive with real-time PCR assay were investigated ‘’precore, core” mutations with the INNO-LiPA method.
HBV DNA was detected as positive in 93 serum samples. In 42 of those 93 (45.2%) serums, precore/core mutations were detected. Isolated precore mutation in 12 cases (12.9%), isolated core mutation in 9 cases (9.7%), and both precore and core mutations in 21 cases (22.6%) were found.
Mutant strains may play an important role in becoming chronic, hepato-carcinogenesis, and in the development of fulminant hepatitis or asymptomatic course. Until valid results are obtained revealing the otherwise, it must be kept in mind that there might be a risk factor of the nucleotide changes in the core region associated with the activation of hepatitis B.
Keywords:  basal core promoter; genotyping techniques; HBeAg; hepatitis B, chronic; hepatitis B core antigens; hepatitis B e antigens; hepatitis B virus; INNO-LiPA; liver diseases; precore/core mutation; promoter regions, genetic
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